The metabolomics platform does metabolomics analyses, including lipidomics, targeted and untargeted by mass spectrometry (gas and liquid chromatography: GC-MS and LC-MS/MS) applied to diverse classes of metabolites: lipids (phospholipids, sphingolipids, triglycerides, etc.), cholesterol, acylcarnitines, bile acids, fatty acids, organic acids and amino acids. We can analyse a variety of biological matrices such as plasma, serum, red blood cells, mitochondria, animal tissues (heart, liver, muscle, brain, etc.) or other species (ex: C. Elgedans) depending on your needs.
We can also do metabolic and functional phenotyping of the mouse heart by its ex-vivo perfusion in working mode in presence of carbon 13 labelled substrates. And we can do metabolic investigations by the analysis of metabolic fluxes based on the utilization of the substrates by stable labelled isotopes and mass spectrometry.
Director : Christine Des Rosiers
Co-director : Matthieu Ruiz
Research assistants and associates:
Bertrand Bouchard, MSc Microbiology: Expertise in mass spectrometry (GC-MS and LC-MS/MS), including sample preparation; Email: bertrand.bouchard@mhi-rc.org
Lise Coderre, PhD Molecular Biology: Expertise in metabolic syndrome and diabetes, molecular signaling; Email: lise.coderre@umontreal.ca
Caroline Daneault, MSc Biochemistry: Expertise in mass spectrometry (GC-MS and LC-MS/MS), results, data and sample analysis/preparation; Email: caroline.daneault@icm-mhi.org
Anik Forest, MSc Physics and Chemistry: Expertise in mass spectrometry (LC-MS/MS) and results analysis (“data mining”); Email: anik.forest@mhi-rc.org
Isabelle Robillard Frayne, MSc Chemistry: Expertise in mass spectrometry (GC-MS and LC-MS/MS), results, data and sample analysis/preparation; Email: isabelle.robillard@mhi-rc.org
Julie Thompson Legault, MSc Nutrition: Expertise in metabolomics and clinical study coordination; Email: julie.thompson@mhi-rc.org
-Lipidomics : untargeted ( ̴1000 unique lipids) / (Semi)-targeted on a chosen class (e.g. ceramides, plasmalogens, phospholipids, cardiolipins, cholesterol and derivatives, etc.)
-Free carnitine and acylcarnitins (C2 to C26)
-Derivatives of the hexosamines pathway
-Amino acids
-Organic acids
-Fatty acids (C14 to C26, including n-3 and n-6)
-Bile acids
-Metabolic fluxes (13C and 2H)
Platform instruments:
-2 LC-QTOF
-1 LC-QQQ
-2 GC-MS
Pubmed : C. Des Rosiers et Matthieu Ruiz
metabolomique.icm@mhi-rc.org
514 376-3330, ext : 2133
Team members
Director : Christine Des Rosiers
Co-director : Matthieu Ruiz
Research assistants and associates:
Bertrand Bouchard, MSc Microbiology: Expertise in mass spectrometry (GC-MS and LC-MS/MS), including sample preparation; Email: bertrand.bouchard@mhi-rc.org
Lise Coderre, PhD Molecular Biology: Expertise in metabolic syndrome and diabetes, molecular signaling; Email: lise.coderre@umontreal.ca
Caroline Daneault, MSc Biochemistry: Expertise in mass spectrometry (GC-MS and LC-MS/MS), results, data and sample analysis/preparation; Email: caroline.daneault@icm-mhi.org
Anik Forest, MSc Physics and Chemistry: Expertise in mass spectrometry (LC-MS/MS) and results analysis (“data mining”); Email: anik.forest@mhi-rc.org
Isabelle Robillard Frayne, MSc Chemistry: Expertise in mass spectrometry (GC-MS and LC-MS/MS), results, data and sample analysis/preparation; Email: isabelle.robillard@mhi-rc.org
Julie Thompson Legault, MSc Nutrition: Expertise in metabolomics and clinical study coordination; Email: julie.thompson@mhi-rc.org
Analytic methods
-Lipidomics : untargeted ( ̴1000 unique lipids) / (Semi)-targeted on a chosen class (e.g. ceramides, plasmalogens, phospholipids, cardiolipins, cholesterol and derivatives, etc.)
-Free carnitine and acylcarnitins (C2 to C26)
-Derivatives of the hexosamines pathway
-Amino acids
-Organic acids
-Fatty acids (C14 to C26, including n-3 and n-6)
-Bile acids
-Metabolic fluxes (13C and 2H)
Platform instruments:
-2 LC-QTOF
-1 LC-QQQ
-2 GC-MS
Publications
Pubmed : C. Des Rosiers et Matthieu Ruiz
Links
Contact
metabolomique.icm@mhi-rc.org
514 376-3330, ext : 2133