Service d'imagerie cellulaire in vitro et ex vivo

L’immunofluorescence observée en microscopie confocale permet de localiser les éléments cellulaires tels que protéines, chaînes lipidiques, ions, et acides nucléiques à l’intérieur de la cellule. Cependant, ce marquage doit être effectué sur des spécimens qui ont été préalablement soumis à une fixation. Alors, les évènements observés sont le reflet d’un seul moment dans le temps sans aucun dynamisme temporel. De plus, le fait que les différents échantillons à comparer soient traités individuellement rend l’analyse quantitative difficile et elle doit être supportée par des expériences biochimiques, électrophysiologiques et de biologie moléculaire.

L’observation confocale en fluorescence sur cellules vivantes permet de visualiser et quantifier avec précision des phénomènes dynamiques dans le temps. Cette visualisation doit s’effectuer sans affecter la viabilité cellulaire, ce qui est primordial dans le but d’obtenir des résultats exacts. Les manipulations doivent donc s’effectuer sous conditions physiologiques similaires à celles présentes in vivo.

Calcium intracellulaire, membranes, organelles, radicaux libres, FRAP, photo-manipulations, protéines fluorescentes, analyses de migration, reconstructions en 4-dimensions, interactions cellules-cellules sont des exemples d’investigations effectuées sur des cellules vivantes avec les microscopes confocaux du Centre de Recherche de l’Institut de Cardiologie de Montréal.

Objectifs
Matériel
Liens
Publications
Prix et distinctions
Contact

Objectifs

Acquisition , traitements et analyse d’images confocale en fluorescence pour les équipes de recherche œuvrant au Centre de Recherche de l’Institut de Cardiologie de Montréal et de leur collaborateurs.
Développement de techniques d’imagerie cellulaire spécifiques pour chaque projet de recherche
Entraînement des chercheurs/étudiants/membres du personnel de recherche aux techniques de marquage en fluorescence, à l’utilisation des microscopes confocaux ainsi qu’aux logiciels d’analyse/reconstruction 3D.

Matériel

Zeiss LSM Duo

Systèmes confocaux combinés soit le LSM 710 et le LSM 5 LIVE (2 détecteurs)
Imagerie en fluorescence de cellules/tissus vivants en conditions physiologiques (incubation, perfusion) ainsi que des cellules/tissus fixés.
Lentilles : 10x/0.3 Plan Neofluar air , 20x/0.8 air , 25x/0.8 LD eau/huile/glycérol, 40x/1.3 Plan Apochromat huile, 63x/1.4 Plan Aprochromat huile, 100x/1.3 Plan Neofluar huile
LASERs : LSM 710 (405 nm-458 nm- 488 nm- 514 nm- 543 nm- 594 nm- 633 nm) et LSM LIVE 5 (405 nm- 488 nm- 561 nm- 635 nm)
“Spectral unmixing”, FRET, imagerie calcique haute vitesse (100 images/secondes), FRAP, libération de peptides encagés, imagerie 3D et 4D
Imagerie en fluorescences multiples.
Photoactivation, photoblanchiment
Images panoramiques-
Imagerie Calciques

Zeiss LSM 510

Objectifs : 10x/0.3 Plan Neofluar air , 20x/0.8 air , 25x/0.8 LD eau/huile/glycérol, 40x/1.3 Plan Apochromat huile, 63x/1.4 Plan Aprochromat huile, 100x/1.3 Plan Neofluar huile.
LASERs : 405 nm-458 nm- 488 nm- 514 nm- 543 nm- 633 nm
Imagerie en fluorescences multiples
Imagerie 3D

Système de microdissection au LASER- Arcturus

LASER 405 nm et 633 nm
Objectifs 2x-10x-20x-40x-60x

Post-acquisition/analyse

Logiciel de déconvolution , d’analyse et de reconstruction en 3 dimensions (3D) Huygens Pro (Scientific Volume Imaging)
Logiciel de reconstruction 3D Volocity (Perkin Elmer)

Liens

Base de données spectrale de l’Université de l’Arizona : http://www.mcb.arizona.edu/IPC/spectra_page.htm
Base de données spectrale de la cie Life Technologies : http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applicat...
http://www.miacellavie.com/MIACellavie/MIACellavie.html
http://www.laboratoirehistologie.org/index.php
http://www.svi.nl/HomePage
http://www.svi.nl/SignalToNoiseRatio#Sample_images_for_an_estimation
http://www.svi.nl/NyquistCalculator

Publications

COAUTEUR

89: Labbé P, Munoz Goyette V, Thorin-Trescases N, Villeneuve L, Desanlis I,
Delwarde C, Shi YF, Martel C, Yu C, Alikashani A, Mamarbachi M, Lesage F,
Mathieu S, Tardif JC, Mathieu P, Kmita M, Thorin É. Angiopoietin-like 2 is
essential to aortic valve development in mice. Commun Biol. 2022 Nov
21;5(1):1277. doi: 10.1038/s42003-022-04243-6. PMID: 36414704.

88: Bonneau S, Landry C, Bégin S, Adam D, Villeneuve L, Clavet-Lanthier MÉ,
Dasilva A, Charles E, Dumont BL, Neagoe PE, Brochiero E, Menaouar A, Nasir B,
Stevens LM, Ferraro P, Noiseux N, Sirois MG. Correlation between Neutrophil
Extracellular Traps (NETs) Expression and Primary Graft Dysfunction Following
Human Lung Transplantation. Cells. 2022 Oct 29;11(21):3420. doi:
10.3390/cells11213420. PMID: 36359815; PMCID: PMC9656095.

87: Rautureau Y, Berlatie M, Rivas D, Uy K, Blanchette A, Miquel G, Higgins MÈ,
Mecteau M, Nault A, Villeneuve L, Lavoie V, Théberge-Julien G, Brand G, Lapointe
L, Denis M, Rosa C, Fortier A, Blondeau L, Guertin MC, Dubé MP, Thorin É, Ledoux
J, Rhainds D, Rhéaume É, Tardif JC. Adenylate cyclase type 9 antagonizes cAMP
accumulation and regulates endothelial signaling involved in atheroprotection.
Cardiovasc Res. 2022 May 16:cvac085. doi: 10.1093/cvr/cvac085. Epub ahead of
print. PMID: 35576489.

86: Bergeron A, Hertig V, Villeneuve L, Chauvette V, El-Hamamsy I, Calderone A.
The ascending aorta of male hypertensive bicuspid aortic valve patients
preferentially associated with a cellular aneurysmal phenotype. Physiol Rep.
2022 Apr;10(8):e15251. doi: 10.14814/phy2.15251. PMID: 35439345.

85 : Amri N, Bégin R, Tessier N, Vachon L, Villeneuve L, Bégin P, Bazin R, Loubaki
L, Martel C. Use of Early Donated COVID-19 Convalescent Plasma Is Optimal to
Preserve the Integrity of Lymphatic Endothelial Cells. Pharmaceuticals (Basel).
2022 Mar 17;15(3):365. doi: 10.3390/ph15030365. PMID: 35337162; PMCID:
PMC8948637.

84: Vachon L, Smaani A, Tessier N, Jean G, Demers A, Milasan A, Ardo N, Jarry S,
Villeneuve L, Alikashani A, Finherty V, Ruiz M, Sorci-Thomas MG, Mayer G, Martel
C. Downregulation of low-density lipoprotein receptor mRNA in lymphatic
endothelial cells impairs lymphatic function through changes in intracellular
lipids. Theranostics. 2022 Jan 1;12(3):1440-1458. doi: 10.7150/thno.58780. PMID:
35154499; PMCID: PMC8771568.

83 : Harel F, Nguyen QT, Nsaibia MJ, Finnerty V, Morgan A, Sirois M, Villeneuve L,
Calderone A, Bergeron A, Brochiero E, Tardif JC, Shi Y, Dupuis J. SPECT imaging
of pulmonary vascular disease in bleomycin-induced lung fibrosis using a
vascular endothelium tracer. Respir Res. 2021 Sep 4;22(1):240. doi:
10.1186/s12931-021-01836-3. PMID: 34481508.

82: Charles E, Dumont BL, Bonneau S, Neagoe PE, Villeneuve L, Räkel A, White M,
Sirois MG. Angiopoietin 1 release from human neutrophils is independent from
neutrophil extracellular traps (NETs). BMC Immunol. 2021 Aug 3;22(1):51. doi:
10.1186/s12865-021-00442-8. PMID: 34344299; PMCID: PMC8336418.

81-Qi XY, Vahdahi Hassani F, Hoffmann D, Xiao J, Xiong F, Villeneuve LR,
Ljubojevic-Holzer S, Kamler M, Abu-Taha I, Heijman J, Bers DM, Dobrev D, Nattel
S. Inositol Trisphosphate Receptors and Nuclear Calcium in Atrial Fibrillation.
Circ Res. 2020 Dec 30. doi: 10.1161/CIRCRESAHA.120.317768. Epub ahead of print.
PMID: 33375812.

80- Bergeron A, Brezai A, Shukr R, Villeneuve L, Allen BG, Qureshi WMS, Hentges
KE, Calderone A. Filamentous nestin and nonmuscle myosin IIB are associated with
a migratory phenotype in neonatal rat cardiomyocytes. J Cell Physiol. 2021
Feb;236(2):1281-1294. doi: 10.1002/jcp.29934. Epub 2020 Jul 11. PMID: 32654195.

79-Emmott A, Hertig V, Bergeron A, Villeneuve L, Lefebvre L, Leask RL, Calderone
A, El-Hamamsy I. Distinct Expression of Nonmuscle Myosin IIB in Pulmonary
Arteries of Patients With Aortic Stenosis vs Insufficiency Undergoing a Ross
Procedure. Can J Cardiol. 2020 Feb 21:S0828-282X(20)30179-3. doi:
10.1016/j.cjca.2020.02.074. Epub ahead of print. PMID: 32544488.

78 -Liu T, Xiong F, Qi XY, Xiao J, Villeneuve L, Abu-Taha I, Dobrev D, Huang C,
Nattel S. Altered calcium-handling produces reentry-promoting action potential
alternans in atrial fibrillation-remodeled hearts. JCI Insight. 2020 Apr 7. pii:
133754. doi: 10.1172/jci.insight.133754. [Epub ahead of print] PubMed PMID:
32255765.

76- Caland L, Labbé P, Mamarbachi M, Villeneuve L, Ferbeyre G, Noly PE, Carrier M,
Thorin-Trescases N, Thorin É. Knockdown of angiopoietin-like 2 induces clearance
of vascular endothelial senescent cells by apoptosis, promotes endothelial repair
and slows atherogenesis in mice. Aging (Albany NY). 2019 Jun 11;11(11):3832-3850.
doi: 10.18632/aging.102020. PubMed PMID: 31186381; PubMed Central PMCID:
PMC6594793.

75-Hertig V, Brezai A, Bergeron A, Villeneuve L, Gillis MA, Calderone A. p38α
MAPK inhibition translates to cell cycle re-entry of neonatal rat ventricular
cardiomyocytes and de novo nestin expression in response to thrombin and after
apex resection. Sci Rep. 2019 Jun 3;9(1):8203. doi: 10.1038/s41598-019-44712-3.
PubMed PMID: 31160695.

74-Pinçon A, De Montgolfier O, Akkoyunlu N, Daneault C, Pouliot P, Villeneuve L,
Lesage F, Levy BI, Thorin-Trescases N, Thorin É, Ruiz M. Non-Alcoholic Fatty
Liver Disease, and the Underlying Altered Fatty Acid Metabolism, Reveals Brain
Hypoperfusion and Contributes to the Cognitive Decline in APP/PS1 Mice.
Metabolites. 2019 May 25;9(5). pii: E104. doi: 10.3390/metabo9050104. PubMed
PMID: 31130652.

73-de Montgolfier O, Pinçon A, Pouliot P, Gillis MA, Bishop J, Sled JG,
Villeneuve L, Ferland G, Lévy BI, Lesage F, Thorin-Trescases N, Thorin É. High
Systolic Blood Pressure Induces Cerebral Microvascular Endothelial Dysfunction,
Neurovascular Unit Damage, and Cognitive Decline in Mice. Hypertension. 2019
Jan;73(1):217-228. doi: 10.1161/HYPERTENSIONAHA.118.12048. PubMed PMID: 30571552.

72-Vigneault P, Naud P, Qi X, Xiao J, Villeneuve L, Davis DR, Nattel S.
Calcium-dependent potassium channels control proliferation of cardiac progenitor
cells and bone marrow-derived mesenchymal stem cells. J Physiol. 2018
Jun;596(12):2359-2379. doi: 10.1113/JP275388. Epub 2018 May 5. PubMed PMID:
29574723; PubMed Central PMCID: PMC6002232.

71-Gélinas R, El Khoury N, Chaix MA, Beauchamp C, Alikashani A, Ethier N, Boucher
G, Villeneuve L, Robb L, Latour F, Mondesert B, Rivard L, Goyette P, Talajic M,
Fiset C, Rioux JD. Characterization of a Human Induced Pluripotent Stem
Cell-Derived Cardiomyocyte Model for the Study of Variant Pathogenicity:
Validation of a KCNJ2 Mutation. Circ Cardiovasc Genet. 2017 Oct;10(5). pii:
e001755. doi: 10.1161/CIRCGENETICS.117.001755. PubMed PMID: 29021306.

70-Hertig V, Matos-Nieves A, Garg V, Villeneuve L, Mamarbachi M, Caland L,
Calderone A. Nestin expression is dynamically regulated in cardiomyocytes during
embryogenesis. J Cell Physiol. 2018 Apr;233(4):3218-3229. doi: 10.1002/jcp.26165.
Epub 2017 Sep 27. PubMed PMID: 28834610; PubMed Central PMCID: PMC5783184

67-Hertig V, Tardif K, Meus MA, Duquette N, Villeneuve L, Toussaint F, Ledoux J,
Calderone A. Nestin expression is upregulated in the fibrotic rat heart and is
localized in collagen-expressing mesenchymal cells and interstitial CD31(+)-
cells. PLoS One. 2017 Apr 27;12(4):e0176147. doi: 10.1371/journal.pone.0176147.
eCollection 2017. PubMed PMID: 28448522; PubMed Central PMCID: PMC5407835.
Maafi F, Li B, Gebhard C, Brodeur MR, Nachar W, Villeneuve L, Lesage F,
Rhainds D, Rhéaume E, Tardif JC. Development of a new bioactivatable fluorescent
probe for quantification of apolipoprotein A-I proteolytic degradation in vitro
and in vivo. Atherosclerosis. 2017 Mar;258:8-19. doi:
10.1016/j.atherosclerosis.2017.01.026. Epub 2017 Jan 21. PubMed PMID: 28167355.

66-Chabot A, Hertig V, Boscher E, Nguyen QT, Boivin B, Chebli J, Bissonnette L,
Villeneuve L, Brochiero E, Dupuis J, Calderone A. Endothelial and Epithelial Cell
Transition to a Mesenchymal Phenotype Was Delineated by Nestin Expression. J Cell
Physiol. 2016 Jul;231(7):1601-10. doi: 10.1002/jcp.25257. Epub 2015 Dec 2. PubMed
PMID: 26574905.

65-Meus MA, Hertig V, Villeneuve L, Jasmin JF, Calderone A. Nestin Expressed by
Pre-Existing Cardiomyocytes Recapitulated in Part an Embryonic Phenotype;
Suppressive Role of p38 MAPK. J Cell Physiol. 2017 Jul;232(7):1717-1727. doi:
10.1002/jcp.25496. Epub 2016 Jul 27. PubMed PMID: 27439108.

64-Poirier S, Hamouda HA, Villeneuve L, Demers A, Mayer G. Trafficking Dynamics
of PCSK9-Induced LDLR Degradation: Focus on Human PCSK9 Mutations and C-Terminal
Domain. PLoS One. 2016 Jun 9;11(6):e0157230. doi: 10.1371/journal.pone.0157230.
eCollection 2016. PubMed PMID: 27280970; PubMed Central PMCID: PMC4900664.

63-Tadevosyan A, Villeneuve LR, Fournier A, Chatenet D, Nattel S, Allen BG. Caged
ligands to study the role of intracellular GPCRs. Methods. 2016 Jan 1;92:72-7.
doi: 10.1016/j.ymeth.2015.07.005. Epub 2015 Jul 18. PubMed PMID: 26196333.

62-Tardif K, Hertig V, Duquette N, Villeneuve L, El-Hamamsy I, Tanguay JF,
Calderone A. Nestin upregulation characterizes vascular remodeling secondary to
hypertension in the rat. Am J Physiol Heart Circ Physiol. 2015 May
15;308(10):H1265-74. doi: 10.1152/ajpheart.00804.2014. Epub 2015 Mar 13. PubMed

61-Tadevosyan A, Létourneau M, Folch B, Doucet N, Villeneuve LR, Mamarbachi AM,
Pétrin D, Hébert TE, Fournier A, Chatenet D, Allen BG, Nattel S. Photoreleasable
ligands to study intracrine angiotensin II signalling. J Physiol. 2015 Feb
1;593(3):521-39. doi: 10.1113/jphysiol.2014.279109. Epub 2015 Jan 7. PubMed PMID:
25433071; PubMed Central PMCID: PMC4324703.
PMID: 25770244.

60-Chabot A, Meus MA, Naud P, Hertig V, Dupuis J, Villeneuve L, El Khoury N,
Fiset C, Nattel S, Jasmin JF, Calderone A. Nestin is a marker of lung remodeling
secondary to myocardial infarction and type I diabetes in the rat. J Cell
Physiol. 2015 Jan;230(1):170-9. doi: 10.1002/jcp.24696. PubMed PMID: 24915827.

59-Tardif K, Hertig V, Dumais C, Villeneuve L, Perrault L, Tanguay JF, Calderone
A. Nestin downregulation in rat vascular smooth muscle cells represents an early
marker of vascular disease in experimental type I diabetes. Cardiovasc Diabetol.
2014 Aug 21;13:119. doi: 10.1186/s12933-014-0119-6. PubMed PMID: 25139503; PubMed
Central PMCID: PMC4143548.

58-Merlen C, Villeneuve LR, Allen BG. Using caged ligands to study intracrine
endothelin signaling in intact cardiac myocytes. Methods Mol Biol.
2015;1234:31-41. doi: 10.1007/978-1-4939-1755-6_4. PubMed PMID: 25304346.

57-Aflaki M, Qi XY, Xiao L, Ordog B, Tadevosyan A, Luo X, Maguy A, Shi Y, Tardif
JC, Nattel S. Exchange protein directly activated by cAMP mediates slow
delayed-rectifier current remodeling by sustained β-adrenergic activation in
guinea pig hearts. Circ Res. 2014 Mar 14;114(6):993-1003. doi:
10.1161/CIRCRESAHA.113.302982. Epub 2014 Feb 7. PubMed PMID: 24508724.

56-Merlen C, Farhat N, Luo X, Chatenet D, Tadevosyan A, Villeneuve LR, Gillis MA,
Nattel S, Thorin E, Fournier A, Allen BG. Intracrine endothelin signaling evokes
IP3-dependent increases in nucleoplasmic Ca²⁺ in adult cardiac myocytes. J Mol
Cell Cardiol. 2013 Sep;62:189-202. doi: 10.1016/j.yjmcc.2013.05.021. Epub 2013
Jun 10. PubMed PMID: 23756157; PubMed Central PMCID: PMC3770605.

55-Vaniotis G, Glazkova I, Merlen C, Smith C, Villeneuve LR, Chatenet D, Therien
M, Fournier A, Tadevosyan A, Trieu P, Nattel S, Hébert TE, Allen BG. Regulation
of cardiac nitric oxide signalling by nuclear β-adrenergic and endothelin
receptors. J Mol Cell Cardiol. 2013 May 16. doi:pii: S0022-2828(13)00171-5.
10.1016/j.yjmcc.2013.05.003. [Epub ahead of print] PubMed PMID: 23684854.

54-Farhat N, Thorin-Trescases N, Mamarbachi M, Villeneuve L, Yu C, Martel C,
Duquette N, Gayda M, Nigam A, Juneau M, Allen BG, Thorin E. Angiopoietin-like 2
promotes atherogenesis in mice. J Am Heart Assoc. 2013 May 10;2(3):e000201. doi:
10.1161/JAHA.113.000201. PubMed PMID: 23666461.

53-El-Helou V, Chabot A, Gosselin H, Villeneuve L, Clavet-Lanthier ME, Tanguay
JF, Enikolopov G, Fernandes KJ, Jasmin JF, Calderone A. Cardiac resident
nestin(+) cells participate in reparative vascularisation. J Cell Physiol. 2013
Mar 4. doi: 10.1002/jcp.24345. [Epub ahead of print] PubMed PMID: 23459851.

52-El-Helou V, Gosselin H, Villeneuve L, Calderone A. The plating of rat scar
myofibroblasts on matrigel unmasks a novel phenotype; the self assembly of
lumen-like structures. J Cell Biochem. 2012 Jul;113(7):2442-50. doi:
10.1002/jcb.24117. PubMed PMID: 22573558.

51-Del Duca D, Tadevosyan A, Karbassi F, Akhavein F, Vaniotis G, Rodaros D,
Villeneuve LR, Allen BG, Nattel S, Rohlicek CV, Hébert TE. Hypoxia in early life
is associated with lasting changes in left ventricular structure and function at
maturity in the rat. Int J Cardiol. 2012 Apr 19;156(2):165-73. doi:
10.1016/j.ijcard.2010.10.135. Epub 2010 Dec 4. PubMed PMID: 21131074.

50-Gendron ME, Thorin-Trescases N, Mamarbachi AM, Villeneuve L, Théorêt JF, Mehri
Y, Thorin E. Time-dependent beneficial effect of chronic polyphenol treatment
with catechin on endothelial dysfunction in aging mice. Dose Response.
2012;10(1):108-19. doi: 10.2203/dose-response.11-014.Thorin. Epub 2011 Oct 14.
PubMed PMID: 22423234; PubMed Central PMCID: PMC3299533.

49-Drouin A, Farhat N, Bolduc V, Thorin-Trescases N, Gillis MA, Villeneuve L,
Nguyen A, Thorin E. Up-regulation of thromboxane A₂ impairs cerebrovascular eNOS
function in aging atherosclerotic mice. Pflugers Arch. 2011 Sep;462(3):371-83.
doi: 10.1007/s00424-011-0973-y. Epub 2011 May 27. PubMed PMID: 21617900.

48-Lauzier B, Merlen C, Vaillant F, McDuff J, Bouchard B, Beguin PC, Dolinsky VW,
Foisy S, Villeneuve LR, Labarthe F, Dyck JR, Allen BG, Charron G, Des Rosiers C.
Post-translational modifications, a key process in CD36 function: lessons from
the spontaneously hypertensive rat heart. J Mol Cell Cardiol. 2011
Jul;51(1):99-108. doi: 10.1016/j.yjmcc.2011.04.001. Epub 2011 Apr 13. PubMed
PMID: 21510957.

47-Béguin PC, El-Helou V, Gillis MA, Duquette N, Gosselin H, Brugada R,
Villeneuve L, Lauzier D, Tanguay JF, Ribuot C, Calderone A. Nestin (+) stem cells
independently contribute to neural remodelling of the ischemic heart. J Cell
Physiol. 2011 May;226(5):1157-65. doi: 10.1002/jcp.22441. PubMed PMID: 20945344.

46-Drouin A, Bolduc V, Thorin-Trescases N, Bélanger É, Fernandes P, Baraghis E,
Lesage F, Gillis MA, Villeneuve L, Hamel E, Ferland G, Thorin E. Catechin
treatment improves cerebrovascular flow-mediated dilation and learning abilities
in atherosclerotic mice. Am J Physiol Heart Circ Physiol. 2011
Mar;300(3):H1032-43. doi: 10.1152/ajpheart.00410.2010. Epub 2010 Dec 24. PubMed
PMID: 21186270.

45-Dingar D, Merlen C, Grandy S, Gillis MA, Villeneuve LR, Mamarbachi AM, Fiset
C, Allen BG. Effect of pressure overload-induced hypertrophy on the expression
and localization of p38 MAP kinase isoforms in the mouse heart. Cell Signal. 2010
Nov;22(11):1634-44. Epub 2010 Jun 17. PubMed PMID: 20600854.

44-Moïse N, Dingar D, Mamarbachi AM, Villeneuve LR, Farhat N, Gaestel M,
Khairallah M, Allen BG. Characterization of a novel MK3 splice variant from
murine ventricular myocardium. Cell Signal. 2010 Oct;22(10):1502-12. Epub 2010
Jun 4. PubMed PMID: 20570725.

Robitaille M, Gora S, Wang Y, Goupil E, Pétrin D, Del Duca D, Villeneuve LR,
Allen BG, Laporte SA, Bernard DJ, Hébert TE. Gbetagamma is a negative regulator
of AP-1 mediated transcription. Cell Signal. 2010 Aug;22(8):1254-66. Epub 2010
Apr 18. PubMed PMID: 20403427.

43-Wakili R, Yeh YH, Qi XY, Greiser M, Chartier D, Nishida K, Maguy A, Villeneuve
LR, Boknik P, Voigt N, Krysiak J, Kääb S, Ravens U, Linke WA, Stienen GJ, Shi Y,
Tardif JC, Schotten U, Dobrev D, Nattel S. Multiple Potential Molecular
Contributors to Atrial Hypocontractility Caused by Atrial Tachycardia Remodeling
in Dogs. Circ Arrhythm Electrophysiol. 2010 Jul 26. [Epub ahead of print] PubMed
PMID: 20660541.

42-Tadevosyan A, Maguy A, Villeneuve LR, Babin J, Bonnefoy A, Allen BG, Nattel S.
Nuclear-delimited angiotensin receptor-mediated signaling regulates cardiomyocyte
gene expression. J Biol Chem. 2010 Jul 16;285(29):22338-49. Epub 2010 May 12.
PubMed PMID: 20463030; PubMed Central PMCID: PMC2903375.

41-Dingar D, Benoit MJ, Mamarbachi AM, Villeneuve LR, Gillis MA, Grandy S,
Gaestel M, Fiset C, Allen BG. Characterization of the expression and regulation
of MK5 in the murine ventricular myocardium. Cell Signal. 2010 Jul;22(7):1063-75.
doi: 10.1016/j.cellsig.2010.02.009. Epub 2010 Mar 7. PubMed PMID: 20214976;
PubMed Central PMCID: PMC3415464.

40-Burstein B, Comtois P, Michael G, Nishida K, Villeneuve L, Yeh YH, Nattel S.
Changes in connexin expression and the atrial fibrillation substrate in
congestive heart failure. Circ Res. 2009 Dec 4;105(12):1213-22. Epub 2009 Oct 29.
PubMed PMID: 19875729.

39-Béguin PC, El-Helou V, Assimakopoulos J, Clément R, Gosselin H, Brugada R,
Villeneuve L, Rohlicek CV, Del Duca D, Lapointe N, Rouleau JL, Calderone A. The
phenotype and potential origin of nestin+ cardiac myocyte-like cells following
infarction. J Appl Physiol. 2009 Oct;107(4):1241-8. Epub 2009 Aug 13. PubMed
PMID: 19679743.

38-Del Duca D, Wong G, Trieu P, Rodaros D, Kouremenos A, Tadevosyan A, Vaniotis
G, Villeneuve LR, Tchervenkov CI, Nattel S, Allen BG, Hébert TE, Rohlicek CV.
Association of neonatal hypoxia with lasting changes in left ventricular gene
expression: an animal model. J Thorac Cardiovasc Surg. 2009 Sep;138(3):538-46,
546.e1. PubMed PMID: 19698832.

37-El-Helou V, Proulx C, Béguin P, Assimakopoulos J, Gosselin H, Clement R,
Villeneuve L, Huot-Marchand JE, Deblois D, Lajoie C, Calderone A. The cardiac
neural stem cell phenotype is compromised in streptozotocin-induced diabetic
cardiomyopathy. J Cell Physiol. 2009 Aug;220(2):440-9. PubMed PMID: 19388005.

36-Sauvageau S, Thorin E, Villeneuve L, Dupuis J. Change in pharmacological
effect of endothelin receptor antagonists in rats with pulmonary hypertension:
role of ETB-receptor expression levels. Pulm Pharmacol Ther. 2009
Aug;22(4):311-7. PubMed PMID: 19489130.

35-Maguy A, Le Bouter S, Comtois P, Chartier D, Villeneuve L, Wakili R, Nishida
K, Nattel S. Ion channel subunit expression changes in cardiac Purkinje fibers: a
potential role in conduction abnormalities associated with congestive heart
failure. Circ Res. 2009 May 8;104(9):1113-22. Epub 2009 Apr 9. PubMed PMID:
19359601.

34-Richer M, David M, Villeneuve LR, Trieu P, Ethier N, Pétrin D, Mamarbachi AM,
Hébert TE. GABA-B(1) receptors are coupled to the ERK1/2 MAP kinase pathway in
the absence of GABA-B(2) subunits. J Mol Neurosci. 2009 May;38(1):67-79. Epub
2008 Dec 4. PubMed PMID: 19052921.

33-Farhat N, Thorin-Trescases N, Voghel G, Villeneuve L, Mamarbachi M, Perrault
LP, Carrier M, Thorin E. Stress-induced senescence predominates in endothelial
cells isolated from atherosclerotic chronic smokers. Can J Physiol Pharmacol.
2008 Nov;86(11):761-9. PubMed PMID: 19011671.

32-El-Helou V, Beguin PC, Assimakopoulos J, Clement R, Gosselin H, Brugada R,
Aumont A, Biernaskie J, Villeneuve L, Leung TK, Fernandes KJ, Calderone A. The
rat heart contains a neural stem cell population; role in sympathetic sprouting
and angiogenesis. J Mol Cell Cardiol. 2008 Nov;45(5):694-702. Epub 2008 Jul 31.
PubMed PMID: 18718475.

31-Sauvageau S, Thorin E, Villeneuve L, Dupuis J. Endothelin-3-dependent
pulmonary vasoconstriction in monocrotaline-induced pulmonary arterial
hypertension. Peptides. 2008 Nov;29(11):2039-45. Epub 2008 Aug 15. PubMed PMID:
18771698.

30-Qi XY, Yeh YH, Xiao L, Burstein B, Maguy A, Chartier D, Villeneuve LR,
Brundel BJ, Dobrev D, Nattel S. Cellular signaling underlying atrial tachycardia
remodeling of L-type calcium current. Circ Res. 2008 Oct 10;103(8):845-54. Epub
2008 Aug 21. PubMed PMID: 18723446.

29-Xiao L, Coutu P, Villeneuve LR, Tadevosyan A, Maguy A, Le Bouter S, Allen BG,
Nattel S. Mechanisms underlying rate-dependent remodeling of transient outward
potassium current in canine ventricular myocytes. Circ Res. 2008 Sep
26;103(7):733-42. Epub 2008 Aug 21. PubMed PMID: 18723449.

28-Voghel G, Thorin-Trescases N, Farhat N, Mamarbachi AM, Villeneuve L, Fortier
A, Perrault LP, Carrier M, Thorin E. Chronic treatment with N-acetyl-cystein
delays cellular senescence in endothelial cells isolated from a subgroup of
atherosclerotic patients. Mech Ageing Dev. 2008 May;129(5):261-70. Epub 2008 Jan
20. PubMed PMID: 18302967.

27-Baragli A, Grieco ML, Trieu P, Villeneuve LR, Hébert TE. Heterodimers of
adenylyl cyclases 2 and 5 show enhanced functional responses in the presence of
Galpha s. Cell Signal. 2008 Mar;20(3):480-92. Epub 2007 Nov 22. PubMed PMID:
18164588.

26-Maliba R, Brkovic A, Neagoe PE, Villeneuve LR, Sirois MG.
Angiopoietin-mediated endothelial P-selectin translocation: cell signaling
mechanisms. J Leukoc Biol. 2008 Feb;83(2):352-60. Epub 2007 Nov 5. PubMed PMID:
17984290.

25-El-Helou V, Proulx C, Gosselin H, Clement R, Mimee A, Villeneuve L, Calderone
A. Dexamethasone treatment of post-MI rats attenuates sympathetic innervation of
the infarct region. J Appl Physiol. 2008 Jan;104(1):150-6. Epub 2007 Oct 4.
PubMed PMID: 17916672.

24-Dupuis J, Préfontaine A, Villeneuve L, Ruel N, Lefebvre F, Calderone A. Bone
marrow-derived progenitor cells contribute to lung remodelling after myocardial
infarction. Cardiovasc Pathol. 2007 Nov-Dec;16(6):321-8. Epub 2007 Jun 20. PubMed
PMID: 18005870.

23-Voghel G, Thorin-Trescases N, Farhat N, Nguyen A, Villeneuve L, Mamarbachi
AM, Fortier A, Perrault LP, Carrier M, Thorin E. Cellular senescence in
endothelial cells from atherosclerotic patients is accelerated by oxidative
stress associated with cardiovascular risk factors. Mech Ageing Dev. 2007
Nov-Dec;128(11-12):662-71. Epub 2007 Oct 13. PubMed PMID: 18022214.

22-Proulx C, El-Helou V, Gosselin H, Clement R, Gillis MA, Villeneuve L,
Calderone A. Antagonism of stromal cell-derived factor-1alpha reduces infarct
size and improves ventricular function after myocardial infarction. Pflugers
Arch. 2007 Nov;455(2):241-50. Epub 2007 May 23. PubMed PMID: 17520275.

21-Gendron ME, Thorin-Trescases N, Villeneuve L, Thorin E. Aging associated with
mild dyslipidemia reveals that COX-2 preserves dilation despite endothelial
dysfunction. Am J Physiol Heart Circ Physiol. 2007 Jan;292(1):H451-8. Epub 2006
Sep 15. PubMed PMID: 16980343.

20-David M, Richer M, Mamarbachi AM, Villeneuve LR, Dupré DJ, Hebert TE.
Interactions between GABA-B1 receptors and Kir 3 inwardly rectifying potassium
channels. Cell Signal. 2006 Dec;18(12):2172-81. Epub 2006 May 23. PubMed PMID:
16809021.

19-Dupré DJ, Robitaille M, Ethier N, Villeneuve LR, Mamarbachi AM, Hébert TE.
Seven transmembrane receptor core signaling complexes are assembled prior to
plasma membrane trafficking. J Biol Chem. 2006 Nov 10;281(45):34561-73. Epub 2006
Sep 7. PubMed PMID: 16959776.

18-Yacoub D, Théorêt JF, Villeneuve L, Abou-Saleh H, Mourad W, Allen BG, Merhi
Y. Essential role of protein kinase C delta in platelet signaling, alpha IIb beta
3 activation, and thromboxane A2 release. J Biol Chem. 2006 Oct
6;281(40):30024-35. Epub 2006 Aug 8. PubMed PMID: 16895913.

17-Zhang Y, Xiao J, Wang H, Luo X, Wang J, Villeneuve LR, Zhang H, Bai Y, Yang
B, Wang Z. Restoring depressed HERG K+ channel function as a mechanism for
insulin treatment of abnormal QT prolongation and associated arrhythmias in
diabetic rabbits. Am J Physiol Heart Circ Physiol. 2006 Sep;291(3):H1446-55. Epub
2006 Apr 14. PubMed PMID: 16617123.

16-Lefebvre F, Préfontaine A, Calderone A, Caron A, Jasmin JF, Villeneuve L,
Dupuis J. Modification of the pulmonary renin-angiotensin system and lung
structural remodelling in congestive heart failure. Clin Sci (Lond). 2006
Sep;111(3):217-24. PubMed PMID: 16640505.

15-Boivin B, Lavoie C, Vaniotis G, Baragli A, Villeneuve LR, Ethier N, Trieu P,
Allen BG, Hébert TE. Functional beta-adrenergic receptor signalling on nuclear
membranes in adult rat and mouse ventricular cardiomyocytes. Cardiovasc Res. 2006
Jul 1;71(1):69-78. Epub 2006 Mar 24. PubMed PMID: 16631628.

14-Calderone A, Bel-Hadj S, Drapeau J, El-Helou V, Gosselin H, Clement R,
Villeneuve L. Scar myofibroblasts of the infarcted rat heart express natriuretic
peptides. J Cell Physiol. 2006 Apr;207(1):165-73. PubMed PMID: 16270351.

13-El-Helou V, Dupuis J, Proulx C, Drapeau J, Clement R, Gosselin H, Villeneuve
L, Manganas L, Calderone A. Resident nestin+ neural-like cells and fibers are
detected in normal and damaged rat myocardium. Hypertension. 2005
Nov;46(5):1219-25. Epub 2005 Oct 17. PubMed PMID: 16230517.

12-Drapeau J, El-Helou V, Clement R, Bel-Hadj S, Gosselin H, Trudeau LE,
Villeneuve L, Calderone A. Nestin-expressing neural stem cells identified in the
scar following myocardial infarction. J Cell Physiol. 2005 Jul;204(1):51-62.
PubMed PMID: 15605421.

11-Boivin B, Villeneuve LR, Farhat N, Chevalier D, Allen BG. Sub-cellular
distribution of endothelin signaling pathway components in ventricular myocytes
and heart: lack of preformed caveolar signalosomes. J Mol Cell Cardiol. 2005
Apr;38(4):665-76. PubMed PMID: 15808843.

10-Migneault A, Sauvageau S, Villeneuve L, Thorin E, Fournier A, Leblanc N,
Dupuis J. Chronically elevated endothelin levels reduce pulmonary vascular
reactivity to nitric oxide. Am J Respir Crit Care Med. 2005 Mar 1;171(5):506-13.
Epub 2004 Dec 3. PubMed PMID: 15579730.

9-Melnyk P, Ehrlich JR, Pourrier M, Villeneuve L, Cha TJ, Nattel S. Comparison
of ion channel distribution and expression in cardiomyocytes of canine pulmonary
veins versus left atrium. Cardiovasc Res. 2005 Jan 1;65(1):104-16. PubMed PMID:
15621038.

8-Fausther M, Villeneuve L, Cadrin M. Heat shock protein 70 expression, keratin
phosphorylation and Mallory body formation in hepatocytes from
griseofulvin-intoxicated mice. Comp Hepatol. 2004 Aug 12;3(1):5. PubMed PMID:
15307891; PubMed Central PMCID: PMC516018.

7-Ehrlich JR, Cha TJ, Zhang L, Chartier D, Villeneuve L, Hébert TE, Nattel S.
Characterization of a hyperpolarization-activated time-dependent potassium
current in canine cardiomyocytes from pulmonary vein myocardial sleeves and left
atrium. J Physiol. 2004 Jun 1;557(Pt 2):583-97. Epub 2004 Mar 12. PubMed PMID:
15020696; PubMed Central PMCID: PMC1665099.

6-Rollin S, Lemieux C, Maliba R, Favier J, Villeneuve LR, Allen BG, Soker S,
Bazan NG, Merhi Y, Sirois MG. VEGF-mediated endothelial P-selectin translocation:
role of VEGF receptors and endogenous PAF synthesis. Blood. 2004 May
15;103(10):3789-97. Epub 2004 Feb 5. PubMed PMID: 14764537.

5-Ledoux J, Greenwood I, Villeneuve LR, Leblanc N. Modulation of Ca2+-dependent
Cl- channels by calcineurin in rabbit coronary arterial myocytes. J Physiol. 2003
Nov 1;552(Pt 3):701-14. Epub 2003 Aug 22. PubMed PMID: 12937294; PubMed Central
PMCID: PMC2343461.

4-Tran Duc AT, Schwab AJ, Simard A, Villeneuve L, Dupuis J. Reduction in
hepatic endothelin-1 clearance in cirrhosis. Clin Sci (Lond). 2003
Aug;105(2):227-34. PubMed PMID: 12708965.

3-Boivin B, Chevalier D, Villeneuve LR, Rousseau E, Allen BG. Functional
endothelin receptors are present on nuclei in cardiac ventricular myocytes. J
Biol Chem. 2003 Aug 1;278(31):29153-63. Epub 2003 May 19. PubMed PMID: 12756260.

2-Jasmin JF, Calderone A, Leung TK, Villeneuve L, Dupuis J. Lung structural
remodeling and pulmonary hypertension after myocardial infarction: complete
reversal with irbesartan. Cardiovasc Res. 2003 Jun 1;58(3):621-31. PubMed PMID:
12798435.

1-Lavoie C, Mercier JF, Salahpour A, Umapathy D, Breit A, Villeneuve LR, Zhu
WZ, Xiao RP, Lakatta EG, Bouvier M, Hébert TE. Beta 1/beta 2-adrenergic receptor
heterodimerization regulates beta 2-adrenergic receptor internalization and ERK
signaling efficacy. J Biol Chem. 2002 Sep 20;277(38):35402-10. Epub 2002 Jul 24.
PubMed PMID: 12140284.

PUBLICATIONS - REMERCIEMENTS ET PARTICIPATION

102: Thibault S, Long V, Fiset C. Higher Na+-Ca2+ Exchanger
Function and Triggered Activity Contribute to Male Predisposition to Atrial
Fibrillation. Int J Mol Sci. 2022 Sep 14;23(18):10724. doi:
10.3390/ijms231810724. PMID: 36142639.

101: Thibault S, Ton AT, Huynh F, Fiset C. Connexin Lateralization Contributes to
Male Susceptibility to Atrial Fibrillation. Int J Mol Sci. 2022 Sep
14;23(18):10696. doi: 10.3390/ijms231810696. PMID: 36142603.

100: Amatullah H, Fraschilla I, Digumarthi S, Huang J, Adiliaghdam F, Bonilla G,
Wong LP, Rivard ME, Beauchamp C, Mercier V, Goyette P, Sadreyev RI, Anthony RM,
Rioux JD, Jeffrey KL. Epigenetic reader SP140 loss of function drives Crohn's
disease due to uncontrolled macrophage topoisomerases. Cell. 2022 Aug
18;185(17):3232-3247.e18. doi: 10.1016/j.cell.2022.06.048. Epub 2022 Aug 10.
PMID: 35952671; PMCID: PMC9442451.

99: Adiliaghdam F, Amatullah H, Digumarthi S, Saunders TL, Rahman RU, Wong LP,
Sadreyev R, Droit L, Paquette J, Goyette P, Rioux JD, Hodin R, Mihindukulasuriya
KA, Handley SA, Jeffrey KL. Human enteric viruses autonomously shape
inflammatory bowel disease phenotype through divergent innate immunomodulation.
Sci Immunol. 2022 Apr 8;7(70):eabn6660. doi: 10.1126/sciimmunol.abn6660. Epub
2022 Apr 8. PMID: 35394816.

98-Mercier V, Boucher G, Devost D, Bourque K, Alikashani A, Beauchamp C, Bitton
A, Foisy S, Goyette P, Charron G, Hébert TE, Rioux JD. IBD-associated G protein-
coupled receptor 65 variant compromises signalling and impairs key functions
involved in inflammation. Cell Signal. 2022 Feb 24;93:110294. doi:
10.1016/j.cellsig.2022.110294. Epub ahead of print. PMID: 35218908.

97- Al Katat A, Zhao J, Calderone A, Parent L. Sympathetic Stimulation
Upregulates the Ca2+ Channel Subunit, CaVα2δ1, via the β1
and ERK 1/2 Pathway in Neonatal Ventricular Cardiomyocytes. Cells. 2022 Jan
6;11(2):188. doi: 10.3390/cells11020188. PMID: 35053304.

96- Boukhatem I, Fleury S, Welman M, Le Blanc J, Thys C, Freson K, Best MG,
Würdinger T, Allen BG, Lordkipanidzé M. The brain-derived neurotrophic factor
prompts platelet aggregation and secretion. Blood Adv. 2021 Sep
28;5(18):3568-3580. doi: 10.1182/bloodadvances.2020004098. PMID: 34546355.

95-Cohesin-protein Shugoshin-1 controls cardiac automaticity via HCN4 pacemaker channel.
Liu D, Song AT, Qi X, van Vliet PP, Xiao J, Xiong F, Andelfinger G, Nattel S.
Nat Commun. 2021 May 5;12(1):2551. doi: 10.1038/s41467-021-22737-5.
PMID: 33953173

94- Cyr Y, Lamantia V, Bissonnette S, Burnette M, Besse-Patin A, Demers A,
Wabitsch M, Chrétien M, Mayer G, Estall JL, Saleh M, Faraj M. Lower plasma PCSK9
in normocholesterolemic subjects is associated with upregulated adipose tissue
surface-expression of LDLR and CD36 and NLRP3 inflammasome. Physiol Rep. 2021
Feb;9(3):e14721. doi: 10.14814/phy2.14721. PMID: 33527668; PMCID: PMC7851436.

93-Le Blanc J, Fleury S, Boukhatem I, Bélanger JC, Welman M, Lordkipanidzé M.
Platelets Selectively Regulate the Release of BDNF, But Not That of Its
Precursor Protein, proBDNF. Front Immunol. 2020 Nov 25;11:575607. doi:
10.3389/fimmu.2020.575607. PMID: 33324399; PMCID: PMC7723927.

92-Segura E, Mehta A, Marsolais M, Quan XR, Zhao J, Sauvé R, Spafford JD, Parent
L. An ancestral MAGUK protein supports the modulation of mammalian voltage-gated
Ca2+ channels through a conserved CaVβ-like interface.
Biochim Biophys Acta Biomembr. 2020 Nov 1;1862(11):183439. doi:
10.1016/j.bbamem.2020.183439. Epub 2020 Aug 16. PMID: 32814116.

91- Long V, Fiset C. Contribution of estrogen to the pregnancy-induced increase
in cardiac automaticity. J Mol Cell Cardiol. 2020 Oct;147:27-34. doi:
10.1016/j.yjmcc.2020.08.005. Epub 2020 Aug 12. PMID: 32798536.

90- Nachar W, Merlet N, Maafi F, Shi Y, Mihalache-Avram T, Mecteau M, Ferron M,
Rhéaume E, Tardif JC. Cardiac inflammation and diastolic dysfunction in
hypercholesterolemic rabbits. PLoS One. 2019 Aug 8;14(8):e0220707. doi:
10.1371/journal.pone.0220707. eCollection 2019. PubMed PMID: 31393963; PubMed
Central PMCID: PMC6687122.

89-Vulesevic B, Lavoie SS, Neagoe PE, Dumas E, Räkel A, White M, Sirois MG. CRP
Induces NETosis in Heart Failure Patients with or without Diabetes.
Immunohorizons. 2019 Aug 9;3(8):378-388. doi: 10.4049/immunohorizons.1900026.
PubMed PMID: 31399487.

88-Nawaito SA, Sahadevan P, Sahmi F, Gaestel M, Calderone A, Allen BG. Transcript
levels for extracellular matrix proteins are altered in MK5-deficient cardiac
ventricular fibroblasts. J Mol Cell Cardiol. 2019 May 16. pii:
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PubMed PMID: 31103477.

87-de Montgolfier O, Pouliot P, Gillis MA, Ferland G, Lesage F, Thorin-Trescases
N, Thorin É. Systolic hypertension-induced neurovascular unit disruption
magnifies vascular cognitive impairment in middle-age atherosclerotic
LDLr(-/-):hApoB(+/+) mice. Geroscience. 2019 May 15. doi:
10.1007/s11357-019-00070-6. [Epub ahead of print] PubMed PMID: 31093829.

86-Milasan A, Smaani A, Martel C. Early rescue of lymphatic function limits
atherosclerosis progression in Ldlr(-/-) mice. Atherosclerosis. 2019
Apr;283:106-119. doi: 10.1016/j.atherosclerosis.2019.01.031. Epub 2019 Jan 31.
PubMed PMID: 30851674

85-Bertrand MJ, Abran M, Maafi F, Busseuil D, Merlet N, Mihalache-Avram T,
Geoffroy P, Tardif PL, Abulrob A, Arbabi-Ghahroudi M, Ni F, Sirois M, L'Allier
PL, Rhéaume É, Lesage F, Tardif JC. In Vivo Near-Infrared Fluorescence Imaging of
Atherosclerosis Using Local Delivery of Novel Targeted Molecular Probes. Sci Rep.
2019 Feb 25;9(1):2670. doi: 10.1038/s41598-019-38970-4. PubMed PMID: 30804367;
PubMed Central PMCID: PMC6389905.

84-Milasan A, Smaani A, Martel C. Early rescue of lymphatic function limits
atherosclerosis progression in Ldlr(-/-) mice. Atherosclerosis. 2019
Apr;283:106-119. doi: 10.1016/j.atherosclerosis.2019.01.031. Epub 2019 Jan 31.
PubMed PMID: 30851674.

83-Rautureau Y, Deschambault V, Higgins MÈ, Rivas D, Mecteau M, Geoffroy P,
Miquel G, Uy K, Sanchez R, Lavoie V, Brand G, Nault A, Williams PM, Suarez ML,
Merlet N, Lapointe L, Duquette N, Gillis MA, Samami S, Mayer G, Pouliot P,
Raignault A, Maafi F, Brodeur MR, Levesque S, Guertin MC, Dubé MP, Thorin É,
Rhainds D, Rhéaume É, Tardif JC. ADCY9 (Adenylate Cyclase Type 9) Inactivation
Protects From Atherosclerosis Only in the Absence of CETP (Cholesteryl Ester
Transfer Protein). Circulation. 2018 Oct 16;138(16):1677-1692. doi:
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82--El Khoury N, Ross JL, Long V, Thibault S, Ethier N, Fiset C. Pregnancy and
oestrogen regulate sinoatrial node calcium homeostasis and accelerate pacemaking.
Cardiovasc Res. 2018 Oct 1;114(12):1605-1616. doi: 10.1093/cvr/cvy129. PubMed
PMID: 29800268; PubMed Central PMCID: PMC6148331

81-Lavoie SS, Dumas E, Vulesevic B, Neagoe PE, White M, Sirois MG. Synthesis of
Human Neutrophil Extracellular Traps Contributes to Angiopoietin-Mediated In
Vitro Proinflammatory and Proangiogenic Activities. J Immunol. 2018 Jun
1;200(11):3801-3813. doi: 10.4049/jimmunol.1701203. Epub 2018 Apr 23. PubMed
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80-Audet N, Dabouz R, Allen BG, Hébert TE. Nucleoligands-repurposing G
Protein-coupled Receptor Ligands to Modulate Nuclear-localized G Protein-coupled
Receptors in the Cardiovascular System. J Cardiovasc Pharmacol. 2018
Apr;71(4):193-204. doi: 10.1097/FJC.0000000000000535. Review. PubMed PMID:
28858907.

79-Mohanan V, Nakata T, Desch AN, Lévesque C, Boroughs A, Guzman G, Cao Z,
Creasey E, Yao J, Boucher G, Charron G, Bhan AK, Schenone M, Carr SA, Reinecker
HC, Daly MJ, Rioux JD, Lassen KG, Xavier RJ. C1orf106 is a colitis risk
gene that regulates stability of epithelial adherens junctions. Science. 2018
Mar 9;359(6380):1161-1166. doi: 10.1126/science.aan0814. Epub 2018 Feb 1. PMID:
29420262; PMCID: PMC6008784

78--Mathieu S, El Khoury N, Rivard K, Paradis P, Nemer M, Fiset C. Angiotensin II
Overstimulation Leads to an Increased Susceptibility to Dilated Cardiomyopathy
and Higher Mortality in Female Mice. Sci Rep. 2018 Jan 17;8(1):952. doi:
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77-Milasan A, Jean G, Dallaire F, Tardif JC, Merhi Y, Sorci-Thomas M, Martel C.
Apolipoprotein A-I Modulates Atherosclerosis Through Lymphatic Vessel-Dependent
Mechanisms in Mice. J Am Heart Assoc. 2017 Sep 22;6(9). pii: e006892. doi:
10.1161/JAHA.117.006892. Erratum in: J Am Heart Assoc. 2017 Nov;6(11):e004194.
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76-Lessard S, Gatof ES, Beaudoin M, Schupp PG, Sher F, Ali A, Prehar S, Kurita R,
Nakamura Y, Baena E, Ledoux J, Oceandy D, Bauer DE, Lettre G. An
erythroid-specific ATP2B4 enhancer mediates red blood cell hydration and malaria
susceptibility. J Clin Invest. 2017 Aug 1;127(8):3065-3074. doi:
10.1172/JCI94378. Epub 2017 Jul 17. PubMed PMID: 28714864; PubMed Central PMCID:
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75-Segura E, Bourdin B, Tétreault MP, Briot J, Allen BG, Mayer G, Parent L.
Proteolytic cleavage of the hydrophobic domain in the Ca(V)α2δ1 subunit improves
assembly and activity of cardiac Ca(V)1.2 channels. J Biol Chem. 2017 Jun
30;292(26):11109-11124. doi: 10.1074/jbc.M117.784355. Epub 2017 May 11. PubMed
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74-Tadevosyan A, Xiao J, Surinkaew S, Naud P, Merlen C, Harada M, Qi X, Chatenet
D, Fournier A, Allen BG, Nattel S. Intracellular Angiotensin-II Interacts With
Nuclear Angiotensin Receptors in Cardiac Fibroblasts and Regulates RNA Synthesis,
Cell Proliferation, and Collagen Secretion. J Am Heart Assoc. 2017 Apr 5;6(4).
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73-Mathieu S, El Khoury N, Rivard K, Gélinas R, Goyette P, Paradis P, Nemer M,
Fiset C. Reduction in Na(+) current by angiotensin II is mediated by PKCα in
mouse and human-induced pluripotent stem cell-derived cardiomyocytes. Heart
Rhythm. 2016 Jun;13(6):1346-54. doi: 10.1016/j.hrthm.2016.02.015. Epub 2016 Feb
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72--Dionne PO, Wener E, Emmott A, Cartier R, Mongrain R, Leask R, El-Hamamsy I.
The Ross procedure: biomechanical properties of the pulmonary artery according to
aortic valve phenotype. Interact Cardiovasc Thorac Surg. 2016 Sep;23(3):371-6.
doi: 10.1093/icvts/ivw148. Epub 2016 May 30. PubMed PMID: 27241051

71-Dayeh NR, Ledoux J, Dupuis J. Lung Capillary Stress Failure and Arteriolar
Remodelling in Pulmonary Hypertension Associated with Left Heart Disease (Group 2
PH). Prog Cardiovasc Dis. 2016 Jul-Aug;59(1):11-21. doi:
10.1016/j.pcad.2016.05.002. Epub 2016 May 16. Review. PubMed PMID: 27195752.

70-Nguyen A, Duquette N, Mamarbachi M, Thorin E. Epigenetic Regulatory Effect of
Exercise on Glutathione Peroxidase 1 Expression in the Skeletal Muscle of
Severely Dyslipidemic Mice. PLoS One. 2016 Mar 24;11(3):e0151526. doi:
10.1371/journal.pone.0151526. eCollection 2016. PubMed PMID: 27010651; PubMed
Central PMCID: PMC4806847.

69-Milasan A, Dallaire F, Mayer G, Martel C. Effects of LDL Receptor Modulation
on Lymphatic Function. Sci Rep. 2016 Jun 9;6:27862. doi: 10.1038/srep27862.
PubMed PMID: 27279328; PubMed Central PMCID: PMC4899717.

68-Poirier S, Mamarbachi M, Chen WT, Lee AS, Mayer G. GRP94 Regulates Circulating
Cholesterol Levels through Blockade of PCSK9-Induced LDLR Degradation. Cell Rep.
2015 Dec 15;13(10):2064-71. doi: 10.1016/j.celrep.2015.11.006. Epub 2015 Nov 25.
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67-Tadevosyan A, Allen BG, Nattel S. Isolation and study of cardiac nuclei from
canine myocardium and adult ventricular myocytes. Methods Mol Biol.
2015;1234:69-80. doi: 10.1007/978-1-4939-1755-6_7. PubMed PMID: 25304349.

66-Demers A, Samami S, Lauzier B, Des Rosiers C, Ngo Sock ET, Ong H, Mayer G.
PCSK9 Induces CD36 Degradation and Affects Long-Chain Fatty Acid Uptake and
Triglyceride Metabolism in Adipocytes and in Mouse Liver. Arterioscler Thromb
Vasc Biol. 2015 Dec;35(12):2517-25. doi: 10.1161/ATVBAHA.115.306032. Epub 2015
Oct 22. PubMed PMID: 26494228.

65-Bou Khzam L, Bouchereau O, Boulahya R, Hachem A, Zaid Y, Abou-Saleh H, Merhi
Y. Early outgrowth cells versus endothelial colony forming cells functions in
platelet aggregation. J Transl Med. 2015 Nov 9;13:353. doi:
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64-Dick M, MacDonald K, Tardif JC, Leask RL. The effect of simvastatin treatment
on endothelial cell response to shear stress and tumor necrosis factor alpha
stimulation. Biomed Eng Online. 2015 Jun 20;14:58. doi:
10.1186/s12938-015-0057-x. PubMed PMID: 26091905; PubMed Central PMCID:
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63-Quang KL, Maguy A, Qi XY, Naud P, Xiong F, Tadevosyan A, Shi YF, Chartier D,
Tardif JC, Dobrev D, Nattel S. Loss of cardiomyocyte integrin-linked kinase
produces an arrhythmogenic cardiomyopathy in mice. Circ Arrhythm Electrophysiol.
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18-Pourrier M, Herrera D, Caballero R, Schram G, Wang Z, Nattel S. The Kv4.2
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Prix et distinctions

2011 : Gagnant au concours d'image en microscopie de la compagnie Scientific Volume imaging
2005 : Gagnant au concours d'image en microscopie confocale de la compagnie Zeiss

Images à la une (Cover pages)

Calderone A. Nestin+ cells and healing the infarcted heart. Am J Physiol Heart
Circ Physiol. 2012 Jan 1;302(1):H1-9. doi: 10.1152/ajpheart.00716.2011. Epub 2011
Oct 14. Review. PubMed PMID: 22003051.

Changes in connexin expression and the atrial fibrillation substrate in congestive heart failure. Burstein B, Comtois P, Michael G, Nishida K, Villeneuve L, Yeh YH, Nattel S.Circ Res. 2009 Dec 4;105(12):1213-22. Epub 2009 Oct 29.

The cardiac neural stem cell phenotype is compromised in streptozotocin-induced diabetic cardiomyopathy. El-Helou V, Proulx C, Béguin P, Assimakopoulos J, Gosselin H, Clement R, Villeneuve L, Huot-Marchand JE, Deblois D, Lajoie C, Calderone A.
J Cell Physiol. 2009 Aug;220(2):440-9

Nestin-expressing neural stem cells identified in the scar following myocardial infarction. Drapeau J. et al, Journal of Cell Physiology, 2005 Juillet;204 (1):51-62.

Chronically elevated endothelin levels reduce pulmonary vascular reactivity to nitric oxide. Migneault A. et al, American Journal of Respiratory and Critical Care Medecine. 2005 Mars 1;171(5):506-13.

Gender- based differencies in cardiac repolarisation in mouse ventricles. Trépanier-Boulay V. et al, Circulation Research, 2001 Août 31;89(5):437-44.

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